Tumor Protein p73 antibody (AA 75-125)

Catalog No. ABIN290398

This Rabbit Polyclonal antibody specifically detects Tumor Protein p73 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Monkey and Hamster.

Quick Overview for Tumor Protein p73 antibody (AA 75-125) (ABIN290398)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivity: Human, Mouse, Rat, Cow, Dog, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tumor Protein p73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Tumor Protein p73 Antibody

Binding Specificity: AA 75-125

Specificity: This peptide sequence is 100 % conserved across multiple p73 isoforms and between rat, mouse, dog and bovine p73. Researchers are encourage to use the the immunogen sequence information and available Bioinformatics data bases to obtain additional information regarding the amino acid sizes associated with various p73 isoforms.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine (100%) Opossum, Chicken, Xenopus (90%) Bat, Rabbit, Turkey, Stickleback, Medaka, Pufferfish, Zebrafish, Nectri, Fusarium (80%).

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide C-THSPYAQPSS corresponding to amino acids 95-104 of human p73, GenBank EAW71468.1. This peptide sequence is 100% conserved across multiple p73 isoforms and between rat, mouse, dog and bovine p73. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine (100%), Opossum, Chicken, Xenopus (90%), Bat, Rabbit, Turkey, Stickleback, Medaka, Pufferfish, Zebrafish, Nectri, Fusarium (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Alternative Name: TP73 / p73

Background: Name/Gene ID: TP73

Synonyms: TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73

Gene ID: 7161

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus