Tumor Protein p73 antibody (AA 2-13)

Catalog No. ABIN290399

The Mouse Monoclonal anti-Tumor Protein p73 antibody has been validated for WB, IHC, IHC (p) and ChIP. It is suitable to detect Tumor Protein p73 in samples from Human and Monkey.

Quick Overview for Tumor Protein p73 antibody (AA 2-13) (ABIN290399)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tumor Protein p73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Clone: 38C674-2

Product Details anti-Tumor Protein p73 Antibody

Binding Specificity: AA 2-13

Specificity: A peptide corresponding to amino acid residues 2-13 LYVGDPARHLAT of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%) Mouse (92%).

Purification: Protein G purified

Immunogen: This antibody was developed against a peptide corresponding to amino acid residues 2-13 LYVGDPARHLAT of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine. Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%), Mouse (92%).

Isotype: IgG1

Application Details

Application Notes: Approved: ChrIP, IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Alternative Name: TP73 / p73

Background: Name/Gene ID: TP73

Synonyms: TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73

Gene ID: 7161

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus