XPO1 antibody (AA 390-408)

Catalog No. ABIN291057

The Rabbit Polyclonal anti-XPO1 antibody (ABIN291057) specifically detects XPO1 in WB, IHC and IHC (p). The antibody is reactive with Human, Cow, Dog, Horse, Chicken, Monkey, Xenopus laevis and Bat samples.

Quick Overview for XPO1 antibody (AA 390-408) (ABIN291057)

Target: XPO1 (Exportin 1 (XPO1))

Reactivity: Human, Cow, Dog, Horse, Chicken, Monkey, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XPO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-XPO1 Antibody

Binding Specificity: AA 390-408

Specificity: Amino acids 390-408 (ASPLLSGSQHFDVPPRRQL) of human Exportin-1 protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Chicken, Platypus, Xenopus (100%) Mouse, Rat, Hamster, Rabbit, Opossum, Zebrafish (95%) Stickleback, Pufferfish (89%).

Purification: Protein G purified

Immunogen: Amino acids 390-408 (ASPLLSGSQHFDVPPRRQL) of human Exportin-1 protein were used as the immunogen. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Chicken, Platypus, Xenopus (100%), Mouse, Rat, Hamster, Rabbit, Opossum, Zebrafish (95%), Stickleback, Pufferfish (89%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for XPO1

Target: XPO1 (Exportin 1 (XPO1))

Alternative Name: XPO1 / CRM1

Background: Name/Gene ID: XPO1

Synonyms: XPO1, CRM1, yeast, homolog, Exp1, Exportin-1, CRM1

Gene ID: 7514

UniProt: O14980

Pathways: M Phase