POLK antibody (AA 817-830)

Catalog No. ABIN291099

Product Details anti-POLK Antibody

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Binding Specificity: AA 817-830

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human POLK.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: POLK (POLK Products)

Synonyms: DINB1 antibody, DINP antibody, POLQ antibody, Dinb1 antibody, POLK antibody, si:ch211-254o18.3 antibody, DNA polymerase kappa antibody, polymerase (DNA directed) kappa antibody, polymerase (DNA directed), kappa antibody, polymerase (DNA directed) kappa L homeolog antibody, POLK antibody, Polk antibody, polk antibody, Tb11.01.0080 antibody, Tb11.01.0010 antibody, Tb11.01.0020 antibody, Tb11.01.0030 antibody, Tb11.01.0040 antibody, Tb11.01.0050 antibody, Tb11.12.0001 antibody, Tb11.12.0002 antibody, Tb11.12.0003 antibody, polk.L antibody, polk-1 antibody

Background: Name/Gene ID: POLK

Synonyms: POLK, DINP, DNA polymerase kappa, DINB1, DINB protein

Gene ID: 51426

UniProt: Q9UBT6

Pathways: DNA Damage Repair