POLK antibody (AA 817-830)

Catalog No. ABIN291099

The Goat Polyclonal anti-POLK antibody (ABIN291099) specifically detects POLK in WB, ELISA, IHC, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for POLK antibody (AA 817-830) (ABIN291099)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLK Antibody

Binding Specificity: AA 817-830

Specificity: Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human POLK.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: POLK

Background: Name/Gene ID: POLK

Synonyms: POLK, DINP, DNA polymerase kappa, DINB1, DINB protein

Gene ID: 51426

UniProt: Q9UBT6

Pathways: DNA Damage Repair