HSP70 antibody

Catalog No. ABIN291560

The Mouse Monoclonal anti-HSP70 antibody is suitable to detect HSP70 in samples from Human, Cow and Monkey. It has been validated for WB, IHC, IP, FACS, IHC (p) and IHC (fro).

Quick Overview for HSP70 antibody (ABIN291560)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Cow, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSP70 Antibody

Specificity: Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species cross-reactivity: Human, monkey, and bovine.

Purification: Purified

Immunogen: HSP70 from HeLa cells.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry (frozen and formalin/paraffin sections), Immunoprecipitation, and Flow Cytometry. Western Blot: 0.5-1 μg/mL (for 2 hours at RT). Immunohistochemistry: 2-4 μg/mL for 30 minutes at RT. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Immunoprecipitation: 2 μg/mg protein lysate. Positive control: HeLa cells, BT474 cells or breast carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70 / Heat Shock Protein 70

Background: Name/Gene ID: HSP70

Synonyms: HSP70