Integrin beta 5 antibody (Extracellular Domain)

Catalog No. ABIN291993

Product Details anti-Integrin beta 5 Antibody

Target: Integrin beta 5 (ITGB5)

Binding Specificity: Extracellular Domain

Reactivity: Human, Mouse, Dog

Host: Sheep

Clonality: Polyclonal

Conjugate: This Integrin beta 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Specificity: Recognizes human Integrin b5 extracellular domain. In Direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rhIntegrin b1, rhIntegrin b3, rhIntegrin b4, rhIntegrin b6, and rmIntegrin b7. Species sequence Homology: mouse, 91 %, canine, 89 %.

Purification: Affinity purified

Immunogen: CHO cell-derived rhIntegrin b5 extracellular domain.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo, ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 0.1-0.2 μg/mL. Detection limit ~5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Immunocytochemistry: 10 μg/mL to detect b5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. Detection limit ~0.2 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized, PBS, 5 % trehalose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Integrin beta 5

Target: Integrin beta 5 (ITGB5)

Alternative Name: ITGB5 / Integrin Beta 5 (ITGB5 Products)

Background: Name/Gene ID: ITGB5
Family: Integrin

Synonyms: ITGB5, Integrin Beta 5, Integrin beta-5, Integrin beta-5 subunit, Integrin, beta 5

Gene ID: 3693

UniProt: P18084

Pathways: Integrin Complex