DPPA4 antibody (AA 125-296)

Catalog No. ABIN292183

This Goat Polyclonal antibody specifically detects DPPA4 in WB, ELISA and ICC. It exhibits reactivity toward Mouse.

Quick Overview for DPPA4 antibody (AA 125-296) (ABIN292183)

Target: DPPA4 (Developmental Pluripotency Associated 4 (DPPA4))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DPPA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-DPPA4 Antibody

Binding Specificity: AA 125-296

Specificity: Selected for its ability to recognize mouse DPPA4 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rhDPPA4 and rmDPPA5.

Purification: Affinity purified

Immunogen: E. coli-derived rmDPPA4 (aa125-296).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 1 ng/lane under non-reducing and reducing conditions. This antibody has not been optimized for Western blot detection of DPPA4 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect mouse DPPA4 in mouse embryonic stem cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 0.2 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for DPPA4

Target: DPPA4 (Developmental Pluripotency Associated 4 (DPPA4))

Alternative Name: DPPA4

Background: Name/Gene ID: DPPA4

Synonyms: DPPA4

Gene ID: 55211

UniProt: Q7L190