BMI1 antibody (AA 252-264)

Catalog No. ABIN292553

The Goat Polyclonal anti-BMI1 antibody is suitable to detect BMI1 in samples from Human. It has been validated for WB, IHC, ELISA, IF, ICC and IHC (p).

Quick Overview for BMI1 antibody (AA 252-264) (ABIN292553)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BMI1 Antibody

Binding Specificity: AA 252-264

Specificity: Amino acids 252-264 of human Bmi1 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human BMI1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:30000), ICC, IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1 / PCGF4

Background: Name/Gene ID: BMI1

Synonyms: BMI1, BMI-1, Flvi-2/bmi-1, FLVI2/BMI1, PCGF4, Polycomb group protein Bmi1, Polycomb complex protein BMI-1, Dna-binding protein bmi-1, Polycomb group ring finger 4, RING finger protein 51, RNF51

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy