GLI3 antibody

Catalog No. ABIN292775

The Goat Polyclonal anti-GLI3 antibody has been validated for WB, ELISA and ICC. It is suitable to detect GLI3 in samples from Human and Mouse.

Quick Overview for GLI3 antibody (ABIN292775)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-GLI3 Antibody

Specificity: Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1 % cross-reactivity with rhGLI-1 and rhGLI-2.

Purification: Affinity purified

Immunogen: E. coli-derived rhGLI-3.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 μg/mL. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI3

Background: Name/Gene ID: GLI3

Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3

Gene ID: 2737

UniProt: P10071

Pathways: Hedgehog Signaling