Kallikrein 13 antibody (AA 19-262)

Catalog No. ABIN292875

Product Details anti-Kallikrein 13 Antibody

Target: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Binding Specificity: AA 19-262

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Kallikrein 13 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Neutralization (Neut)

Specificity: Selected for its ability to recognize human KLK13 in direct ELISAs and Western Blots. In these formats, this antibody shows ~10 % cross-reactivity with rhKallikrein 8, and less than 5 % cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15.

Purification: Affinity purified

Immunogen: NS0-derived rhKLK13 (aa19-262).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP, Neut, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 8.6 μg/mL (IC50) under conditions in which the enzyme was present at 1 μg/mL and the substrate concentration was 100 M. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 Molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for Kallikrein 13

Target: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Alternative Name: KLK13 / Kallikrein 13 (KLK13 Products)

Synonyms: KLK-L4 antibody, KLKL4 antibody, Egfbp-2 antibody, mGk-13 antibody, kallikrein related peptidase 13 antibody, kallikrein related-peptidase 13 antibody, KLK13 antibody, Klk13 antibody

Background: Name/Gene ID: KLK13
Subfamily: Serine S1
Family: Protease

Synonyms: KLK13, Kallikrein 13, Kallikrein-13, KLK-L4, KLKL4, Kallikrein-like protein 4, Kallikrein-like gene 4

Gene ID: 26085

UniProt: Q9UKR3

Pathways: Complement System