BACE2 antibody (AA 63-466)

Catalog No. ABIN293144

The Sheep Polyclonal anti-BACE2 antibody has been validated for WB, ELISA and IP. It is suitable to detect BACE2 in samples from Human.

Quick Overview for BACE2 antibody (AA 63-466) (ABIN293144)

Target: BACE2 (beta-Site APP-Cleaving Enzyme 2 (BACE2))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This BACE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-BACE2 Antibody

Binding Specificity: AA 63-466

Specificity: Selected for its ability to recognize human BACE-2 in direct ELISAs and Western Blots. In these formats, this antibody shows less than 1 % cross-reactivity with rhBACE-1, rhCathepsin D and rhCathepsin E.

Purification: Affinity purified

Immunogen: NS0-derived rhBACE-2 (aa63-466).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human and mouse BACE-2. The detection limit for BACE-2 is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: This antibody has been used to immunoprecipitate rhBACE-2 from conditioned media of transfected NS0 cells. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human and mouse BACE-2. The detection limit for BACE-2 is approximately 0.5 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BACE2

Target: BACE2 (beta-Site APP-Cleaving Enzyme 2 (BACE2))

Alternative Name: BACE2

Background: Name/Gene ID: BACE2
Subfamily: Aspartic A1
Family: Protease

Synonyms: BACE2, 56 kDa aspartic-like protease, AEPLC, ALP56, Aspartic-like protease 56 kDa, APL56, BAE2, Beta-secretase 2, ASP21, CDA13, DRAP, Memapsin-1, Asp 1, ASP1, Aspartyl protease 1, Theta-secretase, Beta secretase 2, CEAP1, Down region aspartic protease

Gene ID: 25825

UniProt: Q9Y5Z0