alpha Tubulin antibody (AA 426-430, AA 426-450)

Catalog No. ABIN293217

This anti-alpha Tubulin antibody is a Mouse Monoclonal antibody detecting alpha Tubulin in WB, IHC, IF, IHC (p), IP and IHC (fro). Suitable for Human, Mouse, Rat, Pig, Cow, Chicken, Guinea Pig and Frog.

Quick Overview for alpha Tubulin antibody (AA 426-430, AA 426-450) (ABIN293217)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Pig, Cow, Chicken, Guinea Pig, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-alpha Tubulin Antibody

Binding Specificity: AA 426-430, AA 426-450

Specificity: Recognizes chicken tubulin alpha (MW 57kD). Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. Does not block microtubule assembly. Does not inhibit polymerization or depolymerization of platelet tubulin in vitro. Blocks (70-80 % ) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species cross-reactivity: bovine, human, porcine, rat, mouse, guinea pig, gerbil and frog.

Purification: Protein G purified

Immunogen: Native chick brain microtubules. Epitope: Localizes between aa 426-450. aa 426-430 are critical for reactivity (2). Cellular Localization: Cytoplasmic.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF, IHC, IHC-Fr, IHC-P, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunohistochemistry (Frozen and formalin/paraffin): 1:200 for 20 min at RT. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate using Protein G. Positive control: LS174T, MAD109 cells. Skin or lung.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha Tubulin

Pathways: Microtubule Dynamics