AIRE antibody (AA 102-115)

Catalog No. ABIN293603

Product Details anti-AIRE Antibody

Target: AIRE (Autoimmune Regulator (AIRE))

Binding Specificity: AA 102-115

Reactivity: Human, Rat, Dog, Horse, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human AIRE. This antibody is expected to recognise isoform 1 (NP_000374.1) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Rat, Horse, Dog, Hamster, Elephant (100%) Panda (93%) Monkey, Mouse, Bovine (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KDVDLSQPRKGRKP from an internal region of human AIRE (NP_000374.1). Percent identity by BLAST analysis: Human, Gorilla, Rat, Horse, Dog, Hamster, Elephant (100%), Panda (93%), Monkey, Mouse, Bovine (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE (AIRE Products)

Synonyms: AIRE1 antibody, APECED antibody, APS1 antibody, APSI antibody, PGA1 antibody, autoimmune regulator antibody, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) antibody, AaeL_AAEL013794 antibody, CpipJ_CPIJ001208 antibody, CpipJ_CPIJ001209 antibody, AIRE antibody, Aire antibody

Background: Name/Gene ID: AIRE

Synonyms: AIRE, APSI, AIRE1, Autoimmune regulator, APECED, APS1, APECED protein, PGA1

Gene ID: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding