AIRE antibody (AA 102-115)

Catalog No. ABIN293603

The Goat Polyclonal anti-AIRE antibody is suitable to detect AIRE in samples from Human, Rat, Dog, Horse and Hamster. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for AIRE antibody (AA 102-115) (ABIN293603)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Rat, Dog, Horse, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AIRE Antibody

Binding Specificity: AA 102-115

Specificity: Human AIRE. This antibody is expected to recognise isoform 1 (NP_000374.1) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Rat, Horse, Dog, Hamster, Elephant (100%) Panda (93%) Monkey, Mouse, Bovine (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KDVDLSQPRKGRKP from an internal region of human AIRE (NP_000374.1). Percent identity by BLAST analysis: Human, Gorilla, Rat, Horse, Dog, Hamster, Elephant (100%), Panda (93%), Monkey, Mouse, Bovine (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE

Background: Name/Gene ID: AIRE

Synonyms: AIRE, APSI, AIRE1, Autoimmune regulator, APECED, APS1, APECED protein, PGA1

Gene ID: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding