EGF antibody

Catalog No. ABIN293787

This anti-EGF antibody is a Sheep Polyclonal antibody detecting EGF in WB and ELISA. Suitable for Rat.

Quick Overview for EGF antibody (ABIN293787)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EGF Antibody

Specificity: Recognizes rat EGF.

Purification: Affinity purified

Immunogen: E. coli-derived recombinant rat Epidermal Growth Factor.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot and ELISA. Western Blot: 0.1-0.2 μg/mL detects ~5 ng/lane under reducing and non-reducing conditions. ELISA (Capture): 0.2-0.8 μg/mL. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, rat EGF antibody and recombinant rat EGF as the standard.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

Gene ID: 1950

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation