HLA-ABC antibody

Catalog No. ABIN294012

This anti-HLA-ABC antibody is a Mouse Monoclonal antibody detecting HLA-ABC in FACS, IP and WB. Suitable for Human.

Quick Overview for HLA-ABC antibody (ABIN294012)

Target: HLA-ABC

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-ABC antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Clone: MEM-147

Product Details anti-HLA-ABC Antibody

Specificity: Reacts with all human classical MHC Class I molecules in native cell-surface forms (e. g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Purification: Protein A purified

Immunogen: PHA-activated peripheral blood lymphocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IP, WB

Usage: Flow Cytometry: Recommended dilution - 1 μg/mL. Western Blotting: Application note - Non-reducing conditions.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HLA-ABC

Target: HLA-ABC

Alternative Name: HLA-A/B/C

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints