Histone H4 antibody (AA 15-30)

Catalog No. ABIN294329

The Rabbit Polyclonal anti-Histone H4 antibody is suitable to detect Histone H4 in samples from Human, Mouse, Rat, Monkey, Chicken, Xenopus laevis, Zebrafish (Danio rerio) and Drosophila melanogaster. It has been validated for WB, IHC and IHC (p).

Quick Overview for Histone H4 antibody (AA 15-30) (ABIN294329)

Target: Histone H4 (HISTH4)

Reactivity: Human, Mouse, Rat, Monkey, Chicken, Xenopus laevis, Zebrafish (Danio rerio), Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H4 Antibody

Binding Specificity: AA 15-30

Specificity: A 14 kD band is observed. The has been evaluated in human, and most likely reacts with additional mammalian, invertebrate, and plant species as the immunogen sequence used to make it is conserved.

Purification: Protein G purified

Immunogen: Synthetic peptide from human Histone H4.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4