HLA-ABC antibody (PE)
Quick Overview for HLA-ABC antibody (PE) (ABIN294418)
Target
See all HLA-ABC AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- Reacts with all human classical MHC Class I molecules in native cell-surface forms (e. g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
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Purification
- Gel filtration
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Immunogen
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PHA-activated peripheral blood lymphocytes.
Type of Immunogen: Cells -
Isotype
- IgG1
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Application Notes
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Approved: Flo (5 μg/mL)
Usage: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 μg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, 15 mM sodium azide, 0.2 % high-grade protease free BSA as a stabilizing agent.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Do not freeze. Product is photosensitive and should be protected from light.
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Storage
- 4 °C
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Storage Comment
- Store at 4°C. Do not freeze. Protect from light.
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- HLA-ABC
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Alternative Name
- HLA-A/B/C
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Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
Target
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