Blood Group Lewis A antibody
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- Target
- Blood Group Lewis A
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
- Specificity
- Recognizes Lewis a blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors
- Purification
- Protein A purified
- Immunogen
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Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Type of Immunogen: Purified protein - Clone
- 7LE
- Isotype
- IgG1
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- Application Notes
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Approved: ELISA, IHC, IHC-P, WB
Usage: Immunohistochemistry (paraffin sections): Recommended dilution - 5 μg/mL. Positive control - colorectal adenocarcinoma. Application note - heat mediated antigen retrieval (sodium citrate). mAb incubation 1 hour / RT. detection DAB. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 15 mM sodium azide, approx, pH 7.4.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- Blood Group Lewis A
- Alternative Name
- Lewis A Blood Group Antigen
- Synonyms
- CD174 antibody, FT3B antibody, FucT-III antibody, LE antibody, Les antibody, fucosyltransferase 3 (Lewis blood group) antibody, FUT3 antibody
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