Plexin A3 antibody (AA 34-150)

Catalog No. ABIN295494

This anti-Plexin A3 antibody is a Goat Polyclonal antibody detecting Plexin A3 in ELISA, IHC and WB. Suitable for Mouse.

Quick Overview for Plexin A3 antibody (AA 34-150) (ABIN295494)

Target: Plexin A3 (PLXNA3)

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Plexin A3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-Plexin A3 Antibody

Binding Specificity: AA 34-150

Specificity: Selected for its ability to recognize mouse Plexin A3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rmPlexin A1, rmPlexin A2, rhPlexin B1, rhPlexin C1, and rhPlexin D1.

Purification: Affinity purified

Immunogen: E. coli-derived recombinant mouse Plexin A3 (aa34-150).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Plexin A3. The detection limit for rmPlexin A3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Plexin A3 in cryosections of E13 mouse embryos. Tissue was fixed overnight in 4 % PFA+14 %picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at room temperature. After blocking, sections were incubated with primary antibody for 3 hours at room temperature followed by NorthernLights 557 donkey anti-goat lgG at room temperature for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Plexin A3. The detection limit for rmPlexin A3 is approximately 1.0 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for Plexin A3

Target: Plexin A3 (PLXNA3)

Alternative Name: PLXNA3 / Plexin A3

Background: Name/Gene ID: PLXNA3

Synonyms: PLXNA3, 6.3, HSSEXGENE, Plexin-A3, SEX, Plexin A3, Plexin-4, PLXN3, PLXN4, XAP-6, Semaphorin receptor SEX

Gene ID: 55558

UniProt: P51805

Pathways: Regulation of Cell Size