ATG4B antibody (AA 350-400)

Catalog No. ABIN295502

The Rabbit Polyclonal anti-ATG4B antibody is suitable to detect ATG4B in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Xenopus laevis and Tetraodontidae (Puffer Fish). It has been validated for WB, IHC and IHC (p).

Quick Overview for ATG4B antibody (AA 350-400) (ABIN295502)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Xenopus laevis, Tetraodontidae (Puffer Fish)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG4B Antibody

Binding Specificity: AA 350-400

Specificity: A portion of amino acid 350-400 of human APG4B.

Purification: Protein G purified

Immunogen: A portion of amino acid350-400of human APG4B.

Type of Immunogen: Protein fragment

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: APG4B / ATG4B

Background: Name/Gene ID: ATG4B

Synonyms: ATG4B, APG4 autophagy 4 homolog B, AUTL1, Autophagin-1, Cysteine protease ATG4B, HAPG4B, APG4B, KIAA0943

Gene ID: 23192

UniProt: Q9Y4P1

Pathways: Autophagy