RGMB antibody

Catalog No. ABIN295544

The Sheep Polyclonal anti-RGMB antibody is suitable to detect RGMB in samples from Human and Mouse. It has been validated for WB, ELISA and IHC.

Quick Overview for RGMB antibody (ABIN295544)

Target: RGMB (RGM Domain Family, Member B (RGMB))

Reactivity: Human, Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This RGMB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RGMB Antibody

Specificity: Selected for its ability to recognize RGM-B in the applications listed. In direct ELISAs and Western Blots, shows less than 5 % cross-reactivity with rmRGM-A and rmRGM-C.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmRGM-B.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse and human RGM-B. The detection limit for rmRGM-B and rhRGM-B is approximately 1 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect mouse RGM-B in cells and tissues. The working dilution is 2-5 μg/mL. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse and human RGM-B. The detection limit for rmRGM-B and rhRGM-B is approximately 0.5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for RGMB

Target: RGMB (RGM Domain Family, Member B (RGMB))

Alternative Name: RGMB

Background: Name/Gene ID: RGMB

Synonyms: RGMB, DRAGON, Repulsive guidance molecule B, RGM domain family member B, RGM domain family, member B

Gene ID: 285704

UniProt: Q6NW40