DLL1 antibody (Extracellular Domain)

Catalog No. ABIN295726

This Sheep Polyclonal antibody specifically detects DLL1 in WB, ELISA and IHC. It exhibits reactivity toward Rat.

Quick Overview for DLL1 antibody (Extracellular Domain) (ABIN295726)

Target: DLL1 (delta-Like 1 (DLL1))

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This DLL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-DLL1 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognize rat DLL1. Species sequence homology: mouse (97 % ), human (91 %), over aa18-537.

Purification: Affinity purified

Sterility: 0.2 μm filtered

Immunogen: NS0-derived rrDLL1 extracellular domain.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Western Blot: 0.1-0.2 μg/mL Detection limit for recombinant rat DLL1 is 5 ng/lane under non-reducing and 25 ng/lane under reducing conditions. Immunohistochemistry: 10 μg/mL Detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4 % PFA+14 %picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 min at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL Detection limit: 0.3 ng/well.~10 % cross-reactivity with rhDLL-1 and >1 % cross reactivity with rrNotch-1.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: DLL1

Background: Name/Gene ID: DLL1

Synonyms: DLL1, Delta-like 1 (Drosophila), Delta (Drosophila)-like 1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, H-Delta-1, DL1, Npa

Gene ID: 28514

UniProt: O00548

Pathways: Notch Signaling, Stem Cell Maintenance