FKBP4 antibody (full length, N-Term)

Catalog No. ABIN2962775

The Mouse Monoclonal anti-FKBP4 antibody (Clone Hi52C) (ABIN2962775) specifically detects FKBP4 in WB, IHC, IP, ICC and FACS. The antibody is reactive with Human, Mouse, Rat, Hamster and Dog samples.

Quick Overview for FKBP4 antibody (full length, N-Term) (ABIN2962775)

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reactivity: Human, Mouse, Rat, Hamster, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This FKBP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: Hi52C

Product Details anti-FKBP4 Antibody

Binding Specificity: full length, N-Term

Specificity: Reacts with human FKBP52. Cross-reacts with mouse, rat, canine and hamster protein.

Purification: Purified (protein G)

Immunogen: Recombinant full-length FKBP52 with an N-terminal polyhistidine tag.

Isotype: IgG1

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
FC(1-2 μg for 10.6 cells) IHC(4 - 5 μg/mL) WB(0.5 - 1 μg/mL)

Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, glycerol 50 % .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium that is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FKBP4

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Alternative Name: FKBP52

Background: FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to transproline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.

Peptidyl-prolyl cis-trans isomerase FKBP4, PPIase FKBP4 51 kDa FK506-binding protein, FKBP51 52 kDa FK506-binding protein, FKBP-52 59 kDa immunophilin, p59 FK506-binding protein 4, FKBP-4 FKBP59 HSP-binding immunophilin, HBI Immunophilin FKBP52 Rotamase Gene name : FKBP4

Gene ID: 2288

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway