IL-33 antibody

Catalog No. ABIN296529

This anti-IL-33 antibody is a Goat Polyclonal antibody detecting IL-33 in WB, ELISA, IHC, FACS and IHC (fro). Suitable for Mouse.

Quick Overview for IL-33 antibody (ABIN296529)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IL-33 Antibody

Specificity: Recognizes mouse IL-33.

Purification: Affinity purified

Immunogen: E. coli-derived rmIL-33.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-Fr (2 - 15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry, ELISA and Immunohistochemistry. Western Blot: 0.1-0.2 μg/mL. Detection limit for rmIL-33 is ~1 ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25 μg/mL Use 10 μL to label 1-5x10^5 cells in a total reaction volume not exceeding 200 μL using activated mouse splenocytes. Immunohistochemistry: 2-15 μg/mL used on frozen mouse intestine samples. Direct ELISA: 0.5-1.0 μg/mL Detection limit for rmIL-33 is ~0.3 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL33

Background: Name/Gene ID: IL33
Family: Interleukin

Synonyms: IL33, C9orf26, DVS27, DVS27-related protein, IL-1F11, IL-33, Interleukin-33, NFEHEV, NF-HEV, Interleukin-1 family member 11, NFHEV, IL1F11, Interleukin 33, RP11-575C20.2

Gene ID: 90865

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response