FHL1 antibody

Catalog No. ABIN7306298

This independently validated Rabbit Polyclonal antibody specifically detects FHL1 in WB, IF and IC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FHL1 antibody (ABIN7306298)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-FHL1 Antibody

Specificity: Recognizes endogenous levels of FHL-1 protein.

Characteristics: Rabbit polyclonal antibody to FHL-1

Purification: The antibody was purified by immunogen affinity chromatography.

Immunogen: Recombinant full length protein of human FHL-1

Application Details

Application Notes: WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)

Restrictions: For Research Use only

Independent Validation (IF)

Validation #101371 (Immunofluorescence)

by: Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.: #101371

Date: 07/19/2017

Antigen: FHL-1

Lot Number: AL1F04A

Method validated: Immunofluorescence

Positive Control: MRL/lpr lupus mice (14 weeks of age)

Negative Control: FH knockout MRL/lpr lupus mice

Notes: Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.

Validation Images

IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.

Full Methods

Primary Antibody: ABIN2966633

Secondary Antibody: goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol:

• Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
• Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
• Sections were cut at 8µm using the cryostat.
• Dry sections for 30min at RT.
• Hydrate sections in PBS 2x for 15min at RT.
• Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
• Wash sections 2x with PBS for 15min at RT.
• Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
• Wash sections 2x for 15min with PBS.
• Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Handling

Format: Liquid

Buffer: Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: FHL-1

Background: SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1

Gene ID: 2273, 14199, 25177

UniProt: Q13642, P97447, Q9WUH4