CD99 antibody (Extracellular Domain)

Catalog No. ABIN296822

The Goat Polyclonal anti-CD99 antibody is suitable to detect CD99 in samples from Human. It has been validated for FACS, WB and ELISA.

Quick Overview for CD99 antibody (Extracellular Domain) (ABIN296822)

Target: CD99

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Product Details anti-CD99 Antibody

Binding Specificity: Extracellular Domain

Specificity: Selected for its ability to recognize human CD99 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rmCD99.

Purification: Affinity purified

Immunogen: NS0-derived rhCD99 extracellular domain.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody has been validated in whole blood CD3+ T cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by a secondary developing reagent such as donkey anti-goat lgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.2 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of Sterile PBS.

Concentration: Lot specific

Buffer: Supplied as a powder lyophilized from a solution containing PBS with 5 % trehalose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background: Name/Gene ID: CD99

Synonyms: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 Molecule, E2 antigen, HBA71

Gene ID: 4267