FPR1 antibody (AA 176-190)

Catalog No. ABIN297066

This anti-FPR1 antibody is a Rabbit Polyclonal antibody detecting FPR1 in WB, IHC and IHC (p). Suitable for Human.

Quick Overview for FPR1 antibody (AA 176-190) (ABIN297066)

Target: FPR1 (Formyl Peptide Receptor 1 (FPR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FPR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FPR1 Antibody

Binding Specificity: AA 176-190

Specificity: The amino acid sequence used as immunogen is 100 % homologous in human, chimp, gorilla and orangutan, and 67 % homologous in mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%) Gibbon (93%) Elephant (80%).

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide corresponding to amino acids 176-190 of human FPR1 (CTFNFSPWTNDPKER), GenBank no. NP_002020.1|. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%), Gibbon (93%), Elephant (80%), Mouse (67%), Rat (47%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for FPR1

Target: FPR1 (Formyl Peptide Receptor 1 (FPR1))

Alternative Name: FPR1 / FPR

Background: Name/Gene ID: FPR1
Subfamily: Chemoattractant
Family: GPCR

Synonyms: FPR1, FPR-1, FMet-Leu-Phe receptor, FMLP, Formyl peptide receptor 1, FPR, N-formyl peptide receptor, FMLP receptor, Fpr receptor, N-formyl peptide receptor 1

Gene ID: 2357

NCBI Accession: NP_002020

UniProt: P21462