Gi alpha 1/2 (AA 345-354) antibody

Catalog No. ABIN297100

This Rabbit Polyclonal antibody specifically detects Gi alpha 1/2 in WB, IHC and IHC (p). It exhibits reactivity toward Rat, Cow, Human and Mouse.

Quick Overview for Gi alpha 1/2 (AA 345-354) antibody (ABIN297100)

Target: Gi alpha 1/2

Reactivity: Rat, Cow, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 345-354

Specificity: Peptide corresponding to amino acids 345 to 354 of Gi alpha1 and 346 to 355 of Gi alpha2

Purification: Protein A/G purified

Immunogen: Synthetic peptide from human Gi Alpha 1/2.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (5 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.08 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details

Target: Gi alpha 1/2