AMPK alpha antibody (pSer485)

Catalog No. ABIN297500

The Rabbit Polyclonal anti-AMPK alpha antibody is suitable to detect AMPK alpha in samples from Human, Mouse, Rat and Monkey. It has been validated for WB.

Quick Overview for AMPK alpha antibody (pSer485) (ABIN297500)

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMPK alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AMPK alpha Antibody

Binding Specificity: pSer485

Specificity: Recognizes endogenous levels of human AMPKa1/a2 only when phosphorylated at serine 485 or serine 491. This antibody does not cross-react with other related proteins. Species cross-reactivity: Mouse, monkey and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser491 of human AMPKa2.

Type of Immunogen: Synthetic phospho-peptide

Application Details

Application Notes: Approved: WB (1:1000)

Usage: Suitable for use in Western Blot. Western Blot: 1:1000. A band is detected at 62kD.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: PRKAA1 + PRKAA2

Background: Name/Gene ID: PRKAA1 / PRKAA2

Synonyms: PRKAA1 / PRKAA2, AMPK1/2, AMPK1/AMPK2

This antibody detects: PRKAA1 + PRKAA2

Pathways: Warburg Effect