SIRT7 antibody (AA 35-51, AA 361-377)

Catalog No. ABIN297655

This Rabbit Polyclonal antibody specifically detects SIRT7 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SIRT7 antibody (AA 35-51, AA 361-377) (ABIN297655)

Target: SIRT7 (Sirtuin 7 (SIRT7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRT7 Antibody

Binding Specificity: AA 35-51, AA 361-377

Brand: IHC-plus™

Specificity: There is no corresponding 40 kD band seen in mouse or rat liver cell lysate.

Purification: Protein G purified

Immunogen: A mixture of synthetic peptides corresponding to amino acids 35-51 and 361-377 of human SIRT7.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SIRT7

Target: SIRT7 (Sirtuin 7 (SIRT7))

Alternative Name: SIRT7 / Sirtuin 7

Background: Name/Gene ID: SIRT7

Synonyms: SIRT7, SIR2L7, Sirtuin type 7, SIR2-like protein 7, Sir2-related protein type 7, Sirtuin 7

Gene ID: 51547

UniProt: Q9NRC8