SOX15 antibody
-
- Target See all SOX15 Antibodies
- SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))
-
Reactivity
- Human
-
Host
-
Sheep
-
Clonality
- Polyclonal
-
Conjugate
- This SOX15 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Specificity
- Selected for its ability to recognize human SOX15 in the applications listed.
- Purification
- Affinity purified
- Immunogen
- E. coli-derived rhSOX15.
- Isotype
- IgG
- Top Product
- Discover our top product SOX15 Primary Antibody
-
-
- Application Notes
-
Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 1 % cross-reactivity with rhSOX1, -2, -5, -6, -7, -10, -12, -14, -15, -17, and rhSOX21. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect SOX15 in NTera2 teratocarcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 0.3 ng/well. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- Lyophilized.
-
- Target
- SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))
- Alternative Name
- SOX15 (SOX15 Products)
- Synonyms
- SOX20 antibody, SOX26 antibody, SOX27 antibody, SRY-box 15 antibody, SRY box 15 antibody, SRY (sex determining region Y)-box 15 antibody, SOX15 antibody, Sox15 antibody
- Background
-
Name/Gene ID: SOX15
Synonyms: SOX15, Protein SOX-15, Protein SOX-20, SOX26, SOX20, Protein SOX-12, SOX27 - Gene ID
- 6665
- UniProt
- O60248
-