Neural Wiskott-Aldrich syndrome protein (WASL) (AA 21-270) antibody

Catalog No. ABIN297874

Product Details

Target: Neural Wiskott-Aldrich syndrome protein (WASL)

Binding Specificity: AA 21-270

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: Selected for its ability to recognize human N-WASP in the applications listed.

Purification: Affinity purified

Immunogen: E. coli-derived rhN-WASP (aa21-270).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 1.0 μg/mL with the appropriate secondary reagents to detect N-WASP. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect human N-WASP on A431 human epidermoid carcinoma cells stimulated with EGF. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human N-WASP. The detection limit for rhN-WASP is approximately 1.0 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details

Target: Neural Wiskott-Aldrich syndrome protein (WASL)

Alternative Name: WASL / N-WASP (WASL Products)

Background: Name/Gene ID: WASL

Synonyms: WASL, N-WASP, NWASP, Wiskott-Aldrich syndrome-like

Gene ID: 8976

UniProt: O00401