IRF3 antibody (AA 206-427)

Catalog No. ABIN297943

This Goat Polyclonal antibody specifically detects IRF3 in WB, ELISA, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for IRF3 antibody (AA 206-427) (ABIN297943)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-IRF3 Antibody

Binding Specificity: AA 206-427

Specificity: Selected for its ability to recognize human IRF3 in the applications listed below. In direct ELISA and Western Blots, this antibody shows ~60 % cross-reactivity with rmIRF3.

Purification: Affinity purified

Immunogen: E. coli-derived rhIRF3 (aa206-427).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 1 μg/mL (w/ appropriate secondary reagents). Detection limit is ~10 ng/lane (non-reducing) and ~1 ng/lane (reducing). Flow Cytometry: 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 HeLa cells in a total reaction volume 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-goat IgG (FITC). Immunocytochemistry: 10 μg/mL detects human IRF3 in HeLa cells. Cells were fixed with PBS, 4 % paraformaldehyde for 20 min at RT and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 min at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1 % BSA. ELISA: 0.5-1.0 μg/mL (w/ appropriate secondary reagents), detection limit is ~0.2 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3 / IRF-3

Background: Name/Gene ID: IRF3

Synonyms: IRF3, Interferon regulatory factor 3, IRF-3

Gene ID: 3661

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades