BIRC7 antibody

Catalog No. ABIN298022

The Mouse Monoclonal anti-BIRC7 antibody is suitable to detect BIRC7 in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for BIRC7 antibody (ABIN298022)

Target: BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BIRC7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 88C570

Product Details anti-BIRC7 Antibody

Specificity: The Livin antibody, clone 88C570 has been shown to recognize both Livin alpha (~39 kD) and Livin beta (~37 kD) isoforms (Nachmias et al, 2003). 2. also recognizes the 30 kD (p30-Livin alpha) and 28 kD (p28-Livin beta) apoptotic cleavage fragments of Livin alpha and beta, respectively (Nachmias et al, 2003). Apoptotic cleavage fragments of Livin have also been referred to as truncated livin (tLivin) (Nachmias et al, 2007). 3. The specificity has been validated using Livin shRNA (Quiping et al, 2004).

Purification: Protein G purified

Immunogen: Recombinant human livin protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for BIRC7

Target: BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))

Alternative Name: BIRC7 / Livin

Background: Name/Gene ID: BIRC7
Family: Apoptosis

Synonyms: BIRC7, KIAP, Livin inhibitor of apoptosis, MLIAP, ML-IAP, RNF50, RING finger protein 50, LIVIN

Gene ID: 79444

UniProt: Q96CA5