Tumor Protein p73 antibody (N-Term)

Catalog No. ABIN298091

The Mouse Monoclonal anti-Tumor Protein p73 antibody has been validated for WB, IHC, IHC (p), IP and ICC. It is suitable to detect Tumor Protein p73 in samples from Human and Mouse.

Quick Overview for Tumor Protein p73 antibody (N-Term) (ABIN298091)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tumor Protein p73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 5B429

Product Details anti-Tumor Protein p73 Antibody

Binding Specificity: N-Term

Specificity: Recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.

Purification: Protein G purified

Immunogen: Full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), IP, WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Alternative Name: TP73 / p73

Background: Name/Gene ID: TP73

Synonyms: TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73

Gene ID: 7161

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus