Fibronectin 1 antibody (Biotin)

Catalog No. ABIN298210

This Rabbit Polyclonal antibody specifically detects Fibronectin 1 in WB, ELISA, IHC, IP and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Cow and Monkey.

Quick Overview for Fibronectin 1 antibody (Biotin) (ABIN298210)

Target: Fibronectin 1 (FN1)

Reactivity: Human, Mouse, Rat, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Fibronectin 1 Antibody (Biotin)

Specificity: Other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross- reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible

Purification: Immunoaffinity purified

Immunogen: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single- band purity was assessed by SDS-PAGE.

Application Details

Application Notes: Approved: ELISA (1:4000 - 1:8000), IHC, IHC-Fr (1:50 - 1:200), IP, WB (1:10000)

Usage: Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 μg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized, 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Target Details for Fibronectin 1

Target: Fibronectin 1 (FN1)

Alternative Name: FN1 / Fibronectin

Background: Name/Gene ID: FN1

Synonyms: FN1, CIG, Cold-insoluble globulin, Fibronectin 1, FINC, LETS, GFND, ED-B, Fibronectin, FN, FNZ, GFND2, Migration-stimulating factor, MSF

Gene ID: 2335

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy