CDHR5 antibody (AA 29-479)
Quick Overview for CDHR5 antibody (AA 29-479) (ABIN298718)
Target
See all CDHR5 AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Binding Specificity
- AA 29-479
-
Specificity
- Recognizes mouse MUCDHL. In direct ELISAs and Western Blots, this antibody shows approximately 5 % cross-reactivity with rhMUCDHL.
-
Purification
- Immunoaffinity purified
-
Immunogen
- Purified, NS0-derived rmMUCDHL (rmMUCDHL, aa 29 - 479, Accession # NP_082345).
-
Isotype
- IgG
-
-
-
-
Application Notes
-
Approved: ELISA, Flo, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Western Blot: Can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for r mMUCDHL is approximately 1 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Tested in flow cytometry using primary kidney cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: Can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for r mMUCDHL is approximately 0.5 ng/well. -
Comment
-
Target Species of Antibody: Mouse
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- deionized water
-
Concentration
- Lot specific
-
Buffer
- Lyophilized.
-
-
- CDHR5 (Mucin and Cadherin-Like (CDHR5))
-
Alternative Name
- CDHR5 / MUCDHL
-
Background
-
Name/Gene ID: CDHR5
Family: Mucin
Synonyms: CDHR5, Mu-protocadherin, Mucin and cadherin-like, MUPCDH, Mucin-like protocadherin, MU-PCDH, MUCDHL, MUCDHL-ALT, MUCDHL-FL -
Gene ID
- 53841
-
NCBI Accession
- NP_082345
-
UniProt
- Q9HBB8
Target
-