CD44 antibody

Catalog No. ABIN298843

This Mouse Monoclonal antibody specifically detects CD44 in FACS, WB, IHC, IHC (p), IP and IHC (fro). It exhibits reactivity toward Human, Baboon and Green Monkey.

Quick Overview for CD44 antibody (ABIN298843)

Target: CD44

Reactivity: Human, Baboon, Green Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 156-3C11

Product Details anti-CD44 Antibody

Specificity: Recognizes human CD44, a 90kD type I transmembrane protein which is otherwise known as HCAM or CD44s. Various isoforms of CD44 exist. The 90kD standard form, recognized by this, is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Antibodies produced by clone 156-3C11 recognize epitope 3, which is a protease resistant epitope on the CD44 Molecule. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

Purification: Affinity purified

Immunogen: Stimulated human leucocytes.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr (1:100), IHC-P (1:100), IP, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. Western Blot: LS-C58044 detects a band of approximately 80kD in HeLa cell lysates. Immunohistology: This product requires

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: Name/Gene ID: CD44

Synonyms: CD44, CDW44, CD44 antigen, Cell surface glycoprotein CD44, CSPG8, ECMR-III, Epican, HUTCH-I, LHR, MDU2, MC56, MIC4, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, PGP-I, Pgp1, Phagocytic glycoprotein I, MDU3, CD44R, HCELL, Heparan sulfate proteoglycan

Gene ID: 960

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling