SLC44A1 antibody (C-Term) (FITC)

Catalog No. ABIN298883

The Mouse Monoclonal anti-SLC44A1 antibody has been validated for FACS, IHC, IP and IHC (fro). It is suitable to detect SLC44A1 in samples from Human.

Quick Overview for SLC44A1 antibody (C-Term) (FITC) (ABIN298883)

Target: SLC44A1 (Solute Carrier Family 44, Member 1 (SLC44A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC44A1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: VIM-15b

Product Details anti-SLC44A1 Antibody (FITC)

Binding Specificity: C-Term

Specificity: Specific for the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70kD multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells. CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine. Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10.

Purification: Affinity purified

Immunogen: MV4-11 acute monocyte leukaemia cells.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo (1:1 - 1:5), IHC, IHC-Fr, IP

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SLC44A1

Target: SLC44A1 (Solute Carrier Family 44, Member 1 (SLC44A1))

Alternative Name: SLC44A1 / CD92

Background: Name/Gene ID: SLC44A1
Subfamily: Choline transporter-like
Family: Transporter

Synonyms: SLC44A1, CD92 antigen, CTL1, RP11-287A8.1, CD92, CDW92, CDW92 antigen, CHTL1

Gene ID: 23446

UniProt: Q8WWI5