NET1 antibody (AA 396-409)

Catalog No. ABIN299308

This Goat Polyclonal antibody specifically detects NET1 in ELISA, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for NET1 antibody (AA 396-409) (ABIN299308)

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This NET1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NET1 Antibody

Binding Specificity: AA 396-409

Specificity: Human NET1. This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Platypus (100%) Orangutan, Rat, Dog, Bat, Hamster, Panda, Horse, Rabbit, Turkey, Chicken (93%) Mouse, Bovine, Pig, Opossum, Stickleback (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DKLEYLDEKQRDPR from an internal region of human NET1 (NP_005854.2, NP_001040625.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Platypus (100%), Orangutan, Rat, Dog, Bat, Hamster, Panda, Horse, Rabbit, Turkey, Chicken (93%), Mouse, Bovine, Pig, Opossum, Stickleback (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NET1

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Alternative Name: NET1

Background: Name/Gene ID: NET1

Synonyms: NET1, ARHGEF8, NET1A, Proto-oncogene p65 Net1

Gene ID: 10276

NCBI Accession: NP_005854, NP_001040625

UniProt: Q7Z628

Pathways: Neurotrophin Signaling Pathway