FRZB antibody (Biotin)

Catalog No. ABIN301675

This Goat Polyclonal antibody specifically detects FRZB in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for FRZB antibody (Biotin) (ABIN301675)

Target: FRZB (Frizzled-Related Protein (FRZB))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FRZB antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FRZB Antibody (Biotin)

Specificity: Recognizes mouse sFRP-3.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmsFRP-3.

Isotype: IgG

Application Details

Application Notes: Approved: IHC (2 - 5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 0.1-0.2 μg/mL, The detection limit is ~5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows ~60 % cross-reactivity with rhsFRP-3. Immunohistochemistry: 2-5 μg/mL, Will detect sFRP-3 in cells and tissues. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL sterile TBS,  pH 7.3, 0.1 % BSA.

Concentration: Lot specific

Buffer: Lyophilized powder from PBS, BSA.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year.

Target Details for FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Alternative Name: SFRP-3 / FRZB

Background: Name/Gene ID: FRZB
Subfamily: Frizzled

Synonyms: FRZB, FRZB-1, FIZ, FRE, FRITZ, Fritz and frizzled, Frizzled homolog-related, FRP-3, FRZB-PEN, HFIZ, FRP, FZRB, SFRP-3, SRFP3, OS1, Frezzled, Frizzled related protein 3, Frizzled-related protein, Frizzled-related protein 1, FRZB1, SFRP3

Gene ID: 2487

UniProt: Q92765

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation