Histone 3 antibody (H3R2me2)

Catalog No. ABIN3017480

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for WB, IF, ChIP, IP and ChIP-seq. It is suitable to detect Histone 3 in samples from Human. There is 1 publication available.

Quick Overview for Histone 3 antibody (H3R2me2) (ABIN3017480)

Target: Histone 3 (H3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3R2me2

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Methylated Antibodies

Purification: Affinity purification

Immunogen: A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200,IP,1:50 - 1:200,ChIP,1:20 - 1:100,ChIP-seq,1:20 - 1:100

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-Histone 3 antibody (ABIN3017480)

Li, Zhu, Wang, Fu, Hou, Ji, Chen, Hu, Shao, Yu, Zhao, Zhang, Du, Bu, Hu, Tang, Zhong, Yang, Zhao, Cen: "Arginine Methyltransferase 1 in the Nucleus Accumbens Regulates Behavioral Effects of Cocaine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 37, pp. 12890-902, (2016) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.,H3.4,H3/g,H3FT,H3t,HIST3H3,Histone H3,HIST1H3A,Signal Transduction,MAPK-Erk Signaling Pathway,MAPK-P38 Signaling Pathway,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Histone H3

Molecular Weight: 15 kDa

Gene ID: 8290

UniProt: Q16695