HLA-DP/DR antibody (Biotin)

Catalog No. ABIN301998

Product Details anti-HLA-DP/DR Antibody (Biotin)

Target: HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DP/DR antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody MEM-136 recognizes a common extracellular epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: PHA-activated peripheral blood lymphocytes.

Clone: MEM-136

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL.

Comment:

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-HLA-DP/DR antibody (Biotin) (ABIN301998)

Koch, Staffler, Hüttinger, Hilgert, Prager, Cerný, Steinlein, Majdic, Horejsí, Stockinger: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." in: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).

Target Details for HLA-DP/DR

Target: HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))

Alternative Name: HLA-DR+DP (HLA-DP/DR Products)