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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) Primary Antibody

GAPDH Reactivity: Human, Mouse, Rat IF, WB Host: Mouse Monoclonal unconjugated
Pubmed (46)
Catalog No. ABIN3020541
$435.60
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  • Target
    GAPDH
    Reactivity
    • 295
    • 184
    • 169
    • 78
    • 67
    • 63
    • 35
    • 35
    • 31
    • 27
    • 23
    • 20
    • 20
    • 19
    • 19
    • 15
    • 13
    • 10
    • 9
    • 8
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 192
    • 115
    • 24
    • 12
    • 6
    • 1
    Mouse
    Clonality
    • 226
    • 123
    Monoclonal
    Conjugate
    • 197
    • 36
    • 24
    • 18
    • 8
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This GAPDH antibody is un-conjugated
    Application
    • 270
    • 134
    • 86
    • 73
    • 51
    • 47
    • 47
    • 38
    • 28
    • 28
    • 21
    • 15
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Western Blotting (WB)
    Purification
    Affinity purification
    Immunogen
    Fusion protein of human GAPDH
    Isotype
    IgG
  • Application Notes
    Recommended dilutions: WB: 1:1000-1:5000, IF: 1:100-1:500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid freeze / thaw cycles
    Storage
    -20 °C/-80 °C
  • Wu, Xiong, Li, Yang, Ge et al.: "Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid ..." in: Molecular & cellular proteomics : MCP, Vol. 17, Issue 1, pp. 146-159, 2019 (PubMed). Method employed by authors: Western Blotting (WB)

    Han, Cai, Gao, Meng, Gao, Wu, Duan, Wang, Dinislam, Lin, Kang, Jiang: "Loss of ATRX suppresses ATM dependent DNA damage repair by modulating H3K9me3 to enhance temozolomide sensitivity in glioma." in: Cancer letters, Vol. 419, pp. 280-290, 2019 (PubMed). Method employed by authors: Western Blotting (WB)

    Zhang, Qian, Zheng, Qian, Wang, Thu, Zhang, Zhou, Tu, Liu, Li, Yang, Li, Xu: "Jujuboside A promotes Aβ clearance and ameliorates cognitive deficiency in Alzheimer's disease through activating Axl/HSP90/PPARγ pathway." in: Theranostics, Vol. 8, Issue 15, pp. 4262-4278, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Yu, Chen, Li, Yang, Zhang, Huangfu, Zheng, Zhao, Lv, Hong, Liang, Shan: "YAP1 contributes to NSCLC invasion and migration by promoting Slug transcription via the transcription co-factor TEAD." in: Cell death & disease, Vol. 9, Issue 5, pp. 464, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Peng, Wang, Shu, Yang, Wang, Ouyang, Shen, Hou, Zhao, Wang, Zhang, Liu, Zhao: "Raddeanin a Suppresses Glioblastoma Growth by Inducing ROS Generation and Subsequent JNK Activation to Promote Cell Apoptosis." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 47, Issue 3, pp. 1108-1121, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Nie, Huang, Deng, Bao, Liu, Feng, Wang, Chen, Qi, Pan: "The purine receptor P2X7R regulates the release of pro-inflammatory cytokines in human craniopharyngioma." in: Endocrine-related cancer, Vol. 24, Issue 6, pp. 287-296, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Wan, Ashraf, Ye, Duan, Zohaib, Wang, Chen, Zhu, Li, Chen, Cao: "MicroRNA-22 negatively regulates poly(I:C)-triggered type I interferon and inflammatory cytokine production via targeting mitochondrial antiviral signaling protein (MAVS)." in: Oncotarget, Vol. 7, Issue 47, pp. 76667-76683, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Wu, Lou, Ge, Xiong: "Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos." in: Scientific reports, Vol. 7, Issue 1, pp. 4022, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Lei, Pan, Li, Zhou, Zhang, He, Peng, Li, Sidhu, Chen, Hua: "H19 regulates the proliferation of bovine male germline stem cells via IGF-1 signaling pathway." in: Journal of cellular physiology, Vol. 234, Issue 1, pp. 915-926, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Dong, Xu, Yang, Shi, Jiang, Chen, Song, Wang: "Associations of Functional MicroRNA Binding Site Polymorphisms in IL23/Th17 Inflammatory Pathway Genes with Gastric Cancer Risk." in: Mediators of inflammation, Vol. 2017, pp. 6974696, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Li, Zhu, Yang, Dib-Hajj, Waxman, Yu, Xu, Cheng: "Nav1.7 is phosphorylated by Fyn tyrosine kinase which modulates channel expression and gating in a cell type-dependent manner." in: Molecular pain, Vol. 14, pp. 1744806918782229, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Zhang, Zhou, Huang, Yu, Zhang, Wang: "Placental mechanism of prenatal nicotine exposure-reduced blood cholesterol levels in female fetal rats." in: Toxicology letters, Vol. 296, pp. 31-38, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Sheng, Yu, Chen, Wang, Lu: "Infection of grass carp reovirus induced the expressional suppression of pro-viral Fibulin-4 in host cells." in: Fish & shellfish immunology, Vol. 77, pp. 294-297, 2018 (PubMed). Method employed by authors: ELISA (ELISA)

    Zhang, Zhang, Deng, Zhang, Liu, Xin, Li, Xu: "Baicalin attenuates non-alcoholic steatohepatitis by suppressing key regulators of lipid metabolism, inflammation and fibrosis in mice." in: Life sciences, Vol. 192, pp. 46-54, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Liu, Song, Zhao, He, Wang, Chen, Xiang, Yi, Qi, Liu: "Antitumor Activity and Mechanism of a Reverse Transcriptase Inhibitor, Dapivirine, in Glioblastoma." in: Journal of Cancer, Vol. 9, Issue 1, pp. 117-128, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Liu, Zhang, Yao, Xie, Dai, Zhang, Zhou: "Serum extracellular vesicles promote proliferation of H9C2 cardiomyocytes by increasing miR-17-3p." in: Biochemical and biophysical research communications, Vol. 499, Issue 3, pp. 441-446, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Li, Zhou, Yang, Li, Zhang, Wang, Jiao: "SGK1 inhibits PM2.5-induced apoptosis and oxidative stress in human lung alveolar epithelial A549 cells." in: Biochemical and biophysical research communications, Vol. 496, Issue 4, pp. 1291-1295, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Ruan, Zhao, Zhao, Zhang, Shi, Pang: "Knockdown of long noncoding RNA MEG3 impairs VEGF-stimulated endothelial sprouting angiogenesis via modulating VEGFR2 expression in human umbilical vein endothelial cells." in: Gene, Vol. 649, pp. 32-39, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Qiu, Wu, Meng, Luo, Zhao, Sun, Gao: "GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic mice." in: The Journal of surgical research, Vol. 225, pp. 29-39, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Shen, Zhu, Kang, Tu, Li, Zhang, Qin, Yang, Guan: "Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract." in: Journal of ophthalmology, Vol. 2018, pp. 4125893, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

  • Target
    GAPDH
    Alternative Name
    GAPDH (GAPDH Antibody Abstract)
    Synonyms
    G3PD, GAPD, Gapd, BEST:GH12586, CG12055, Dmel\\CG12055, GA3PDH, GADPH, GAP, GAPDH, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, g3pd, G3PDH, glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase 1, glyceraldehyde-3-phosphate dehydrogenase S homeolog, glyceraldehyde-3-phosphate dehydrogenase, type I, olfactory receptor 8K3, GAPDH, Gapdh, Gapdh1, gapdh, gapdh.S, gapDH, LOC100404960, LOC614985
    Background
    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
    Synonyms: GAPDH, G3PD, GAPD, MGC88685
    Gene ID
    2597
    UniProt
    P04406
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