TXN antibody

Catalog No. ABIN3020582

This Mouse Monoclonal antibody specifically detects TXN in WB. It exhibits reactivity toward .

Quick Overview for TXN antibody (ABIN3020582)

Target: TXN (Thioredoxin (TXN))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This TXN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TXN Antibody

Purification: Affinity purification

Immunogen: Recombinant full length protein Trx

Isotype: IgG

Application Details

Application Notes: Recommended dilutions: WB: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C/-80 °C

Target Details for TXN

Target: TXN (Thioredoxin (TXN))

Alternative Name: Trx

Background: Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx has several biological functions. It acts as a hydrogen donor for ribonucleotide reductase, which is critical for DNA synthesis, and modulates the DNA-binding activity of several transcription factors, including NFkappaB, AP-1, p53, TFIIIC and glucocorticoid receptor. Trx also stimulates cell growth, is an inhibitor of apoptosis and plays a role in the protection against oxidative stress. Drugs that inhibit Trx have antitumor activity, suggesting that Trx is involved in a variety of human diseases, including cancer. Thioredoxin 2 (Trx-2) is a small redox protein that is localized to the mitochondria and is essential for cell viability, playing a crucial role in the scavenging of ROS in mitochondria and regulating the mitochondrial apoptosis signaling pathway. Trx reductases (TrxR1 and TrxR2) are ubiquitously expressed flavoproteins that catalyze the NADPH-dependent reduction of Trx as well as several other oxidized cellular components. Mammalian Trx reductases are a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. TrxR1 and TrxR2 are also involved in the prevention of oxidative stress. Inhibition of TrxR activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases.

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis