INPP5D antibody (N-Term)

Catalog No. ABIN302082

The Mouse Monoclonal anti-INPP5D antibody (Clone SHIP-02) (ABIN302082) specifically detects INPP5D in WB, ICC and ICFC. The antibody is reactive with Human samples.

Quick Overview for INPP5D antibody (N-Term) (ABIN302082)

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This INPP5D antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone: SHIP-02

Product Details anti-INPP5D Antibody

Binding Specificity: N-Term

Purpose: Anti-SHIP1 Purified

Cross-Reactivity (Details): Human, Other not determined

Characteristics: The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells (intracellular antigen). Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Intracellular staining, recommended dilution: 2-5 μg/mL, positive control: human blood leukocytes.
Western blotting: Positive control: RAMOS human cell line, reducing conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for INPP5D

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Alternative Name: SHIP1

Background: Inositol polyphosphate-5-phosphatase D,SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.,IPP5PD, IP5PD, INPP5D

Gene ID: 3635

UniProt: Q92835

Pathways: TCR Signaling, BCR Signaling, Warburg Effect