For best experience we recommend to activate Javascript in your browser.

Mouse anti-Human IgG (Fc Region) Antibody

Name: Mouse anti-Human IgG (Fc Region) Antibody
Brand: antibodies-online
SKU: ABIN302085
Availability: InStock

ELISA, FACS, WB, ICC Monoclonal EM-07 IgG1 unconjugated

IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B). (Mouse anti-Human IgG (Fc Region) Antibody)

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC). (Mouse anti-Human IgG (Fc Region) Antibody)

FACS

Separation of human monocytes (red-filled) from CD3 negative IgG (Fc) positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC). (Mouse anti-Human IgG (Fc Region) Antibody)

FACS

Flow cytometry multicolor surface staining of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood). (Mouse anti-Human IgG (Fc Region) Antibody)

FACS

5 images

Catalog No. ABIN302085

$341.39

Plus shipping costs $50.00

100 μg

Shipping to: United States

Delivery in 5 to 9 Business Days

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (ABIN302085)

Target

IgG

Clonality

Monoclonal

Application

2569
2478
2270
1814
1471
918
871
404
357
232
232
103
91
85
48
42
33
24
19
18
16
16
12
9
8
8
5
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1

ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC)

Clone

EM-07

Isotype

IgG1

Binding Specificity
2898

374

346

154

131

88

67

29

28

28

17

5

5

2

1

1

1

1

1

1

1

1

1

1
Fc Region
Reactivity
777

735

653

436

434

244

208

205

194

154

123

118

99

97

81

76

70

58

28

27

27

27

27

16

10

9

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1
Human
Host
1800

1730

609

405

149

95

30

25

20

20

18

12

11
Mouse

Purpose

Anti-Hu IgG (Fc) Purified

Specificity

The mouse monoclonal antibody EM-07 reacts with Fc part of human heavy chain of secreted IgG antibodies and with isolated Fc fragments. It does not react with IgG Fc domain in a complex of BCR (B cell antigen receptor).

Cross-Reactivity (Details)

Human, Other not determined. The antibody EM-07 does not crossreact with IgM.

Characteristics

The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Immunogen

Fusion protein of human IgG Fc fragment.
Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody unconjugated

Mouse anti-Human IgG (Fab Region) antibody (PE)
FACS Monoclonal 4A11 IgG1 PE

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Mouse anti-Human IgG (Fc Region) antibody (HRP)
Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody HRP

Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG Texas Red (TR)

Goat anti-Human IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB Polyclonal IgG unconjugated

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Human IgG (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)
ELISA, WB Polyclonal IgG beta-Gal

Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)

Application Notes

Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Target

IgG

Target Type

Antibody

Background

Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment

Molecular Weight

150 kDa

Gene ID

35