HRASLS2 antibody (Internal Region)

Catalog No. ABIN302144

The Goat Polyclonal anti-HRASLS2 antibody has been validated for ELISA and IHC. It is suitable to detect HRASLS2 in samples from Human.

Quick Overview for HRASLS2 antibody (Internal Region) (ABIN302144)

Target: HRASLS2 (HRAS-Like Suppressor 2 (HRASLS2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HRASLS2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-HRASLS2 Antibody

Binding Specificity: Internal Region

Purpose: HRASLS2

Sequence: QELPYSLTSD NC

Specificity: This design was based on provisional sequence data.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QELPYSLTSDNC, from the internal region of the protein sequence according to NP_060348.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows a granular pattern in the cytoplasm of hepatocytes. Recommended concentration, 5-10 μg/mL.

Western Blot: Preliminary experiments gave an approx. 21 kDa band in Human Kidney lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HRASLS2

Target: HRASLS2 (HRAS-Like Suppressor 2 (HRASLS2))

Alternative Name: HRASLS2

Background: HRASLS2 , HRAS-like suppressor 2, FLJ20556

Gene ID: 54979

NCBI Accession: NP_060348