Resistin Like beta (RETNLB) (Internal Region) antibody

Details for Product No. ABIN302148
Antigen
  • FIZZ1
  • FIZZ2
  • HXCP2
  • RELM-beta
  • RELMb
  • RELMbeta
  • XCP2
  • RETNLB
  • 9030012B21Rik
  • Fizz2
  • Relmb
  • Xcp3
  • resistin like beta
  • RETNLB
  • Retnlb
Epitope
Internal Region
13
11
10
7
6
6
6
5
3
3
2
1
1
1
Reactivity
Human
115
57
2
Host
Goat
142
12
10
6
Clonality
Polyclonal
Conjugate
Un-conjugated
23
7
6
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC)
129
114
34
13
8
6
5
4
4
3
3
2
1
1
1
1
1
Options
Purpose RELMbeta
Immunogen Peptide with sequence C-DSVMDKKIKDVLNS, from the internal region of the protein sequence according to NP_115968.1.
Blocking Peptide Blocking peptide for this product available: ABIN368933
Sequence DSVMDKKIKD VLNS
Isotype IgG
Predicted Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name RETNLB (RETNLB Antibody Abstract)
Background RETNLB , resistin like beta , FIZZ1, FIZZ2, HXCP2, RELM-beta, RELMb, RELMbeta, XCP2, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2, colon and small intestine-specific cysteine-rich protein, cysteine-rich secreted A12-alpha-like protein 1, found in inflammatory zone 1
Gene ID 84666
NCBI Accession NP_115968
Pathways Hormone Activity
Application Notes

DS WB Results: Preliminary experiments gave an approx 35 kDa band in Human Ileum and Duodenum lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7 kDa according to NP_115968.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

DS IHC Results: In paraffin embedded Human Colon shows textured staining of cytoplasm in enterocytes. Recommended concentration, 2-4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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