ATG5 antibody (Internal Region)

Catalog No. ABIN302179

Product Details anti-ATG5 Antibody

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: ELISA

Purpose: ATG5

Sequence: SQVINEMQKK DHKQ

Cross-Reactivity: Cow, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Recent

Immunogen: Peptide with sequence C-SQVINEMQKKDHKQ, from the internal region of the protein sequence according to NP_004840.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx. 28-30 kDa in lysate of cell line MDA-MB-231, after 1 μg/mL antibody staining (calculated Mwt. Of 32.4 kDa according to NP_004840.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5 (ATG5 Products)

Synonyms: APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, ATG5 antibody, CG1643 antibody, DmAtg5 antibody, Dmel\\CG1643 antibody, atg5 antibody, 2010107M05Rik antibody, 3110067M24Rik antibody, AW319544 antibody, Apg5l antibody, Atg5l antibody, C88337 antibody, Paddy antibody, apg5l antibody, zgc:100934 antibody, ATATG5 antibody, AUTOPHAGY 5 antibody, MKP11.20 antibody, MKP11_20 antibody, autophagy related 5 antibody, Autophagy-related 5 antibody, ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, autophagy related 5 L homeolog antibody, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 antibody, autophagy protein Apg5 family antibody, ATG5 antibody, Atg5 antibody, atg5 antibody, atg5.L antibody, APG5 antibody

Background: ATG5, ATG5 autophagy related 5 homolog (S. cerevisiae), APG5, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae)-like, APG5 autophagy 5-like, ATG5 autophagy related 5-like, OTTHUMP00000040507, apoptosis specific protein

Gene ID: 9474, 11793

NCBI Accession: NP_004840

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy